Radioimmunoassay of Plasma Aldosterone and its Clinical Application

A simplified radioimmunoassay of plasma aldosterone.

The analytical evaluation is described of a [125I]-aldosterone radioimmunoassay method for measuring aldosterone in human plasma, which requires no chromatographic purification before quantification by radioimmunoassay but does require organic solvent extraction. Rabbit anti-aldosterone serum is used, generated against aldosterone-3-oxime coupled to thyroglobulin. The antibody demonstrates negl...

Quantitation of plasma aldosterone by radioimmunoassay.

Aldosterone antibodies were produced by injecting sheep with the albumin conjugate of aldosterone. 18,21-dihemisuccinate. Conditions optimal for an accurate, reproducible assay with this antiserum were determined experimentally. Aldosterone is extracted from plasma and isolated by a single thin.layer chromatographic system before the immunoassay is performed. Normal plasma aldosterone in supine...

construction and validation of translation metacognitive strategy questionnaire and its application to translation quality

like any other learning activity, translation is a problem solving activity which involves executing parallel cognitive processes. the ability to think about these higher processes, plan, organize, monitor and evaluate the most influential executive cognitive processes is what flavell (1975) called “metacognition” which encompasses raising awareness of mental processes as well as using effectiv...

Radioimmunoassay of IgE and IgE antibody and its clinical application.

Improved extraction of aldosterone from plasma and urine before radioimmunoassay.

Radioimmunoassay of urinary or plasma aldosterone requires an initial extraction of steroids before purification and analysis. This extraction characteristically must be performed on acid-hydrolyzed urine or plasma, both of which have been supplemented with tnitiated aldosterone, to assess the analytical recovery. After extraction, the organic phase must be washed sequentially with 0.1 mol/lite...